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The analysis of the phosphorylation status of these targeted molecules in the different CRC stages showed increased activation leading to the identification of the IGF system as a key regulator in CRC progression ( in vitro , by inhibiting IGF1R expression highlighting their tumor suppressor activity in CRC, the importance of IGF1R in CRC pathogenesis, and underlying a novel regulatory network to fine-tune cell proliferation ( A further study identified a group of 77 miRNAs that were differentially expressed between early and late time points of inflammation and tumor growth in a mouse model of colitis-associated cancer, histologically and molecularly similar to human CRC ( in silico analysis highlighted that these miRNAs were predicted to target genes involved in the PI3K/AKT and IGF1 signaling cascades

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