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After three washes with PBS containing 0.5% Tween-20, the membranes were incubated with a diluted horseradish peroxidase-conjugated secondary anti-rabbit (GE Healthcare, Buckinghamshire, UK) at room temperature for 2 h
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51 The long-term gene expression has also been observed with AAV9-lncExACT1 driven by a cardiac-specific troponin promoter showing upregulated lncExACT1 even at 16 weeks post injection which induced pathological cardiac hypertrophy
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