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Peptides were collected by centrifugation, desalted with C18 StageTips, and quantified with OD280

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Well-supported claims: GHK-Cu stimulates collagen, elastin, and glycosaminoglycan synthesis in cell culture GHK-Cu accelerates wound healing in animal models GHK-Cu modulates expression of thousands of genes involved in tissue repair and antioxidant defense Topical GHK-Cu produces measurable improvements in skin firmness, wrinkle depth, and collagen density in clinical studies Claims that need more evidence: Optimal concentration for topical formulations (most studies used different concentrations) Whether palmitoylated variants deliver equivalent in vivo effects to native GHK-Cu Long-term safety data for daily topical use beyond 12 weeks How GHK-Cu compares head-to-head with retinoids, the gold standard for anti-aging skincare Whether GHK-Cu's gene expression effects measured in vitro translate to meaningful changes when applied topically Claims without current support: Systemic anti-aging effects from topical application Cancer prevention (despite anti-DNA-damage gene upregulation in cell culture) Hair regrowth from GHK-Cu specifically (AHK-Cu is the variant studied for hair) The clinical trials are generally small, short-term, and sometimes lack proper controls