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Blockade of BRD4–HiBiT degradation by MLN4924 and carfilzomib was not observed at higher concentrations approaching 10 µM, which we suspect is because of the transcriptional consequences of BET bromodomain inhibition, as JQ1 itself decreases BRD4–HiBiT signal at these high concentrations (Extended Data Fig

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Thus, we found that mesenteric arterioles of homozygous mice (GPx1 −/− ) and older heterozygous (GPx1 +/− ) knockout mice had a loss of NO-dependent vasodilatory responses to β- methacholine and bradykinin, instead showing a vasoconstrictor response to these mediators that cause vasodilation in wildtype arterioles [39, 71]